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pTEN

Phosphatase and tensin homolog; PTEN; MMAC1

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Antigen Preparation

A synthetic peptide derived from C-terminus of human pTEN protein.

Background

"Thephosphatase and tensin homologue (PTEN) tumor suppressoris a phosphatidylionositol D3-phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. PTEN is mutated in a wide range of cancers.In vitro, the recombinant PTEN can be regulated by phosphorylation on multiple serine and threonine residues in its C-terminus, such as CK2, GSK3beta at Ser-362, 370 and 385. It antagonizes signal transduction downstream of PI-3 kinases affecting cell proliferation, apoptosis and migration. Loss-of-function mutations in the PTEN gene lead to constitutive activation of multiple signaling pathways."

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY This antibody recognizes IRAK-4(pT345) with a phosphorylated site at Threonine 345. It does not cross-react with non-phosphospecific peptide.
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

Not tested

Order Info

Catalog #:	602-970
Lot #:	See the label
Size:	100ug/200ul
Host:	Mouse
Clone:	M10_KZ
Isotyping:	IgG1
Applications:	IHC(P), WB
Reactivity:	Hu, Ms, Rt
Price:	$ 245.00

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