Antigen Preparation
A synthetic peptide corresponding to N-terminus of SARS-Cov2-Nucleocapsid Protein
Background
"SARS-CoV-2, which causes the COVID-19 disease, is a novel coronavirus which results in an acute respiratory syndrome. It is an enveloped positive-sense RNA virus. SARS-CoV-2 contains four structural proteins: E (envelope), M (membrane), S (spike), and N (nucleocapsid) proteins. The N protein holds the RNA genome, and the S, E, and M proteins form the viral envelope. Coronavirus S protein is a large, multifunctional class I viral transmembrane protein. It is required for the entry of the virion particles into the cell through a contact with host cellular receptors. The S-protein of coronavirus consists of S1 and S2 functional units. S1 is responsible for host receptor binding, S2 is in charge of fusion. The nucleocapsid (N) is a 45 kDa protein, one of the major structural proteins of the virus. It binds directly to viral RNA and provides stability. N-protein is involved in the transcription and viral RNA replication, packaging of the genome into helical ribonucleocapsid, and interference with cell cycle processes of host cells."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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