Antigen Preparation
A recombinant protein of human CD122
Background
"The interleukin 2 receptor is composed of alpha and beta subunits. The beta subunit (CD122) encoded by this gene is very homologous to the human beta subunit and also shows structural similarity to other cytokine receptors. The protein encoded by this gene is a type I membrane protein. IL-2 receptor β chain (IL-2Rβ) interacts with the CIS, a cytokine-inducedSH2-containing protein. Targeting CD122 is the novel approach for clinical optimization of costimulatory blockade strategies in transplantation. Studies show that costimulation-independent T cell alloreactivity relies on signaling through CD122, the shared IL-2 and IL-15 receptor β-chain. Combined costimulatory and CD122 blockade improved survival of transplanted tissue in mice and nonhuman primates by controlling proliferation and effector function of CD8+ T cells. Other studies suggest that the IL2R beta-chain induces growth potential for glandular epithelial cells and an immune-privileged condition mediated by CD25+regulatory-T cells. Frequent and spontaneous proliferation of glandular epithelium and dilated cysts were shown in the uteri of interleukin-2 receptor (IL2R) beta-chain transgenic (Tg2Rbeta) mice. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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