Antigen Preparation
Enriched cell membrane of human PBMC
Background
"CD45 leucocyte common antigen (LCA) belongs to the family of membrane glycoproteins expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues.This protein is a member of the protein tyrosine phosphatase (PTP) family. CD45 can exist in at least nine different isoforms resulting from tissue-specific alternative RNA splicing of exons 4-7 of a single gene coding for the various N-terminal peptide segments. A point mutation within CD45 exon A is the cause of variant CD45RA splicing in humans. CD45 isoforms show cell-type and differentiation-stage specific expression, a pattern which is quite well conserved in mammals. CD45RA is located on naïve T cells and when they are activated or transformed into memory cells, CD45RA is lost. Naive T lymphocytes are typically positive for CD45RA. Tregs, like other T cells, can be divided into CD45RA+ naïve and CD45RA− memory subtypes. CD45RA is a 220 kDa molecule, a single chain type I glycoprotein. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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