Antigen Preparation
A recombinant protein of human CD235a
Background
"CD235a (Glycophorin A, GYPA) is member of the glycophorin A family. It is a 10 kD type I sialoglycoprotein. Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which carries the antigenic determinants for the MN and Ss blood groups. CD235a is expressed by erythrocytes and erythroid precursors. The human MN blood group antigens are isoforms of glycophorin A (GPA) encoded by the gene, GYPA, and are the most abundant erythrocyte sialoglycoproteins. The distribution of MN antigens has been widely studied in human populations yet the evolutionary and/or demographic factors affecting population variation remain elusive. While the primary function of GPA is yet to be discovered, it serves as the major binding site for the 175-kD erythrocyte-binding antigen (EB-175) of the malarial parasite, Plasmodium falciparum. More specifically, exon two of GYPA encodes the receptor-binding ligand to which P. falciparum binds. Several other viruses bind to CD235a"
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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