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SSEA-4

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Antigen Preparation

A recombinant protein SSEA-4

Background

"The antibody SSEA-4 (Stage-specific embryonic antigen 4) MC813-70 was raised against human teratocarcinoma cell line. This monoclonal antibody reacts with a glycolipid carbohydrate epitope on SSEA-4 protein. SSEA-4, a cell surface antigen, is a well-known marker of embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells. In human cells, these surface markers are down-regulated during the differentiation process. Glycosphingolipids, such as the globo-series GSLs stage-specific embryonic antigen 3 (SSEA-3), SSEA-4, and Globo-H, are known to be associated with various biological processes such as cell recognition, cell adhesion, and signal transduction. SSEA-4 is also frequently overexpressed in cancer cells and cancer stem cells. "

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY This antibody recognizes human SSEA-4 protein. The other species are not tested.
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

Not tested

Order Info

Catalog #:	606-280
Lot #:
Size:	100ug/200ul
Host:	Mouse
Clone:	MC813-70
Isotyping:	IgG3
Applications:	IHC(P), WB
Reactivity:	Hu
Price:	$ 245.00

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