Antigen Preparation
A recombinant protein consisting of the C-terminal region (~150aa) of human Occludin
Background
"Occludin is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mounting evidence demonstrated that occludin is not only present in epithelial/endothelial cells, but is also found in large amounts in cells that do not have tight junctions but have very active metabolism. Astrocytes and neurons express the tight junction-specific protein occludin in vitro. Occludin is an enzyme that oxidizes NADH. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Altered occludin expression has been linked to dedifferentiation and cancer progression and invasiveness. Occludin is overexpressed in Alzheimer's disease and vascular dementia."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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