Antigen Preparation
"A synthetic peptide corresponding to the Internal sequence (from 80aa-120aa) of human SOX14 protein. This sequence is identical in human, mouse and rat species."
Background
"The forkhead box gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. FOXA1 plays an important role in breast cancer. The FOXA subfamily is composed of the transcription factors FOXA1, A2, A3. They were discovered as the regulators of hepatic genes. The sumoylation of Foxa1 is observed in several breast cancer cell lines. Foxa1 is modified by sumoylation on lysine, K6, and the SUMO modification of Foxa1 modulates the activity of Foxa1 on its target genes in breast cancer cells."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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