EWSR
EWSR1; Ewing sarcoma breakpoint region 1
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Antigen Preparation
"A synthetic peptide corresponding to the internal segment of EWSR1 protein. This sequence is identical among human, rat, mouse and dog species."
Background
"The EWSR1 gene encodes a putative RNA binding protein. Mutation in this gene causes Ewing sarcoma and neuroectodermal and various other tumors. This gene is involved in a number of different sarcomas as a result of chromosomal translocations. EWSR1 is fused to an ETS transcription factor (FLI-1, ERG, ETV-1, E1AF or FEV) in Ewing sarcoma; to activating transcription factor-1 (ATF-1) in soft tissue clear cell sarcoma; to Wilms Tumor -1 (WT1) in desmoplastic small round cell; to nuclear receptor 4A3 (NR4A3) in extraskeletal myxoid chondrosarcoma, and to C/EBP-homologous protein (CHOP) in myxoid liposarcoma. EWSR1 genes are required for mitotic integrity and survival of neural cells in the early embryonic development, and that the involvement of EWSR1 in mitotic stability is conserved from zebrafish to human."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody recognizes ~70 kDa of EWSR1 protein. It also cross reacts with human, mouse and rat tissues. The other species are not tested. "
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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