Antigen Preparation
A synthetic peptide derived from internal segment of human c-Myc
Background
"The MYC family of proto-oncogenes consists of three members: c-myc (MYC), l-myc (MYCL), and n-myc (MYCN). C-Myc is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Myc genes are essential for normal development as well as for homeostasis of individual tissues, including intestine, skin and the hematopoietic compartment. Expression of MYC proteins is enhanced and deregulated in many human tumors. Mutations, overexpression, rearrangement and translocation of Myc gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma, in human. There are different causes for enhanced MYC expression in individual tumors, which include alterations in the MYC genes themselves, or amplifications of MYC family genes. Deregulated expression of MYC contributes to tumorigenesis and is required to maintain tumor growth."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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