Antigen Preparation
A synthetic peptide corresponding to internal sequence (within 300-360aa) of human Saposin.
Background
"The Saposin family includes four structurally related proteins, saposin A, B, C, and D which are ~80 aa small acidic acitvator proteins with specificities for glycosphingolipid (GSL) hydrolases. They are derived from a single precursor, prosaposin. In both humans and mice, prosaposin/saposin deficiencies lead to severe neurological deficits. Saposins are processed from prosaposin in the late endosome and lysosome, and their stability is maintained by three disulfide bridges. The post-modified prosoposin is an ~70 kDa form which subsequently undergoes partial proteolysis to produce saposin A, B, C, and D, each proteins are between 12-14 kDa in mass. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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