Antigen Preparation
"A synthetic peptide derived from the adjacent to C-terminus of JAK1 protein. This sequence is identical in JAK1 from human, rat and mouse origins."
Background
"The Janus tyrosine kinase family is consists of four members, JAK1, JAK2, JAK3 and Tyk2 ranging from 120-140 kDa. Upon the cytokine ligand binding to its the receptors, the dimerization of the receptors occurs followed by phosphorylation of the receptors. Jak1 plays an essential and nonredundant role in promoting biologic responses induced by a select subset of cytokine receptors. Appropriate activation of JAK1/STAT3 signaling is necessary for cell proliferation, survival, and differentiation, but persistent STAT3 activation may result in disease. JAK1/STAT3 pathway is one of the recognized oncogenic signaling pathways that frequently overactivated in a variety of human tumors. Somatic mutations in JAK1 gene occur in individuals with acute lymphoblastic leukemia (ALL). STAT3 and both JAK1 and 2 are involved in human colorectal cancer cell growth, survival, invasion, and migration through regulation of gene expression."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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