Antigen Preparation
A synthetic peptide derived from internal segment of human STAT5b
Background
"STAT proteins (Signal transducer and activator of transcription) belong to a family of cytoplasmic transcription factors that can be phosphorylated by a lignad binding to its cell surface receptor. Activation of STAT5a induces dimerization, nuclear translocation and DNA binding. Stat5b is activated by IL-2, IL-7, IL-15, IL-3, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin; activation and dimerization of Stat5b requires phosphorylation of STATs."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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