Antigen Preparation
The Synthetic peptide corresponding to the N-term of human Nanog (29aa-50aa).
Background
"Embryonic stem (ES) cells derived from the inner cell mass (ICM) of blastocysts grow infinitely while maintaining pluripotency. Leukemia inhibitory factor (LIF) can maintain self-renewal of mouse ES cells through activation of Stat3. However, LIF/Stat3 is dispensable for maintenance of ICM and human ES cells, suggesting that the pathway is not fundamental for pluripotency. Nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells and ICM. Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct4 and other transcription factors such as KLF4, c-Myc and Lin28."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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