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SSEA4
Stage-specific embryonic antigen 4 (SSEA-4)

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Antigen Preparation
A synthetic peptide derived from the C-terminus of human SSEA-4 protein.
Background
"SSEA-4 is stage-specific embryonic antigen 4 (SSEA4) is a cell surface antigen, expressed along with SSEA3, TRA1-60 and TRA1-81 in embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and up-regulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody recognizes human SSEA4 protein. The other species are not tested.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 620-500
Lot #: See the label
Size: 100ug/200ul
Host: Rabbit
Clone:
Isotyping: Rabbit IgG
Applications: IHC(P), WB
Reactivity: Hu
Price: $ 245.00
REFERENCES
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