Histone H3
HIST1H3A

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Antigen Preparation
A synthetic peptide within ~100 amino acids from N-terminus of human histone H3.
Background
"Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4. The sequence is found in all mammals and a wide range of species, including D. melanogaster, Arabidopsis, Chicken and Xenopus. "
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
SPECIFICITY
This antibody only recognizes ~ 17 kDa of Histone H3 protein.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 620-360
Lot #: See the label
Size: 100ug/200ul
Host: Rabbit
Clone:
Isotyping: Rabbit IgG
Applications: IHC(P), WB, IP
Reactivity: Hu, Ms, Rt, Ck, Bv
Price: $ 199.00
REFERENCES
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