Antigen Preparation
A synthetic peptide of DYKDDDDK conjugated to a carrier protein
Background
"Many oligo peptides or tag proteins are widely used as a tag to monitor the protein expression, such as FLAG, c-Myc, E-tag, His-tag, GFP, GST, and etc. These tag antibodies provide tools to localize gene products in a variety of cell types to study the topology of proteins, and protein complexes, and to identify associated proteins. In addition, this antibody allows characterizing of newly identified, lowing abundance or poorly immunogenic proteins when protein specific antibodies are not available. The FLAG antibody is generated according to the 8 amino acid hydrophilic epitope tag (DYKDDDDK), and it can be used to detect the FLAG tagged fusion proteins. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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