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SP1

Sp1 transcription factor

Antigen Preparation

A recombinant protein derived from 150-200 aa of human SP1

Background

Sp1 is a sequence-specific transcription factor that recognizes GGGGCGGGGC and closely related sequences, which are often referred to as GC boxes. Sp1 belongs to a subgroup of transcription factors that are phosphorylated upon binding to promoter sequences.

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY This antibody recognizes ~90 kDa of human SP1 protein. The other species are not tested.
FORMULATION This affinity purified antibody is supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.	STORAGE The antibodies are stable for 12 months from date of receipt when stored at –20oC. The antibodies can be stored at 2oC-8oC for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

6667

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

5-10 µg/ml

Order Info

Catalog #:	605-830
Lot #:	See the label
Size:	100ug
Host:	Mouse
Clone:	SP1
Isotyping:	IgG1
Applications:	ELISA, WB, IHC
Reactivity:	Hu
Price:	$ 285.00

REFERENCES

Wei-Guo Zhu; et al. Methylation of Adjacent CpG Sites Affects Sp1/Sp3 Binding and Activity in the p21Cip1 Promoter . Molecular and Cellular Biology, June 2003, p. 4056-4065, Vol. 23, No. 12