Antigen Preparation
A chromatography purified from pigmented melanoma metastases from lymph nodes.
Background
"Metastatic melanoma is often confused with a variety of poorly differentiated carcinomas, sarcomas, and large cell lymphomas. Clone HMB45 reacts with fetal and neonatal melanocytes but not with normal adult melanocytes, junctional nevus cells but not with intradermal nevi, hence showing specificity for detection of melanocytic tumors. The panel of tumor markers, most commonly used in conjunction with HMB45, for evaluation of melanoma includes S-100 protein LCA, CEA, and EMA, as well as vimentin, an intermediate filament found in both melanomas and sarcomas."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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