Phosphotyrosin

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Antigen Preparation
A chemically linked phosphotyrosine.
Background
"Protein phosphorylation is involved in cell signaling pathways. These cascades are mediated by three types of kinases: serine, threonine and tyrosine kinases which phosphorylate serine, threonine and tyrosine amino acid side chains.These three amino acids are phosphorylated by its specific kinases. These processes are regulated by kinases and phosphatases."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
"This antibody recognizes Phosphotyrosin. It corss reacts to human, mice, rat. The other species are not tested."
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 603-830
Lot #: See the label
Size: 100ug/200ul
Host: Mouse
Clone: 7E11
Isotyping: IgG1
Applications: IHC(P), WB
Reactivity: Hu, Ms, Rt, Bv
Price: $ 245.00
REFERENCES
"Trinidad JC, Thalhammer A, Specht CG, Lynn AJ, Baker PR, Schoepfer R, Burlingame AL. Quantitative analysis of synaptic phosphorylation and protein expressio. Mol. Cell Proteomics 7 (4): 684–96, 2008"
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