Desmoglein 3
DSG3, Desmoglein 3 (Dsg3); Pemphigus Vulgaries Antigen (PVA)
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Antigen Preparation
The recombinant protein of human Desmoglein-3 extracellular domain.
Background
"Dsg3 and Dsg1 are members of the desmosomal cadherins that provide adhesive integrity to desmosomes between adjoining keratinocytes; the former distributes primarily in the deeper epidermis, whereas the latter is localized to the superficial epidermis. This distribution profile of these target antigens,Dsg3 and Dsg1, of PV (Pemphigus Vulgaris ) and pemphigus foliaceus autoantibodies explains the characteristic clinical features and histopathological localization of acantholysis in their respective diseases. Dsg3 plays important roles in cell-cell adhesion in PV-autoimmune disease as well as in human squamous cell cancer. The level of Dsg3 can be used as a read-out of pathogenic changes."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
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SPECIFICITY
This antibody recognizes human Desmoglein 3 protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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