Sialyltransferase (Biotinylated)
ST6Gal
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Antigen Preparation
A synthetic peptide derived from the internal domain of Sialyltransferase protein from drosophila origin
Background
Sialyltransferases are enzyme that transfer sialic acid to nascent oligosaccharide. Each silaytransferase is specific for a particular sugar substrate. Sialytransferases add sialic acid to the terminal portins of the sialylated glycolipids or to the N-or O-linked sugar chases of glycoproteins. The animal sialyltransferases are Golgi type II transmembrane glycosyltransferases. While the enzyme ST6Gal I uses the Galß1-4GlcNAc-R as the acceptor substrate which present in insects.
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes ~51 kDa of drosophila Sialytransferase protein. The other species are not tested.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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