Antigen Preparation
A synthetic peptide corresponding to the N-terminus of human Cytokeratin protein.
Background
"Cytokeratin-19 is one member of the type A (acidic) subfamily of low molecular weight keratins. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I (A) cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 staining in breast cancer distinguishes malignant from benign tumors. Keratin 19 is often co-expressed with keratin 7."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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