Antigen Preparation
"A twenty-one amino acids synthetic peptide corresponding to the internal sequence of HIF alpha protein. This sequence is identical to human, rat and mouse origins."
Background
"The hypoxia-inducible transcription factor 1 alpha (HIF-1 alpha) contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a transcription factor involved in mammalian oxygen homeostasis., HIF-1 is a heterodimer composed of HIF-1 alpha/beta subunits which binds to hypoxia-response elements (HREs) in the promoters of many genes involved in the adaptation to an environment of insufficient oxygen or hypoxia. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation, and increase transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the hypoxic cells. In contrast, HIF-1 beta remains stable under either condition. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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