Antigen Preparation
"A synthetic peptide corresponding to the C-terminus of human FOXP3 protein. This sequence is identical within human, mouse, rat origins."
Background
"Forkhead/winged-helix box (Fox) genes are the transcriptional regulators and highly conserved across mammals. It contains a FOX DNA-binding domain and large polyglutamine tract. The FOXPs are involved in organogenesis and cell differentiation. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, at low level in CD4 positive/CD25 negative cells, and is absent in CD4 negative/CD8 positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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