pTEN (Paired S380)
Phosphatase and tensin homolog; PTEN; MMAC1
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Antigen Preparation
"A synthetic peptide surrounding to the epitope -DTSDP—without a phosphorylation site at Ser385 of human PTEN protein. This sequence is identical among human, mouse, rat , bovine and chicken origins."
Background
"The phosphatase and tensin homologue (PTEN) tumor suppressor is a phosphatidylinositol D3-phosphatase that is frequently mutated in tumor cells. PTEN is itself regulated by phosphorylation on multiple serine and threonine residues in its C terminus. Casein kinase 2 (CK2) and Glycogen synthase kinase 3beta (GSK3beta), ATK/PI3K are involved in PTEN phosphorylation at sites of S370, S380, T382, T383, S385. The PTEN phosphorylation may play critical roles in the tumor suppressor functions. "
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes PTEN (Paired S385) without a phosphorylation site at Serine 385. It does not cross-react with phosphospecific peptides.
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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