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GAPDH

glyceraldehyde-3-phosphate dehydrogenase

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Antigen Preparation

A purified rabbit muscle GAPDH (whole molecule)

Background

"GAPDH, also known as glyceraldehyde-3-phosphate dehydrogenase, is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases. "

PURIFICATION The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY "This antibody recognizes Glyceraldehyde-3-PDH monomer (36 kDa) and the dimer form. It reacts with human, rabbit, rat, mouse, dog, cat, fish and pig. It does not react with GAPDH from bovine, goat and yeast. "
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

2597

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

Not tested

Order Info

Catalog #:	602-010
Lot #:	See the label
Size:	100ug/200ul
Host:	Mouse
Clone:	258
Isotyping:	IgG1
Applications:	IHC(P), WB
Reactivity:	Hu
Price:	$ 245.00

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