Coagulation Factor VIII (Cleaved form, C-Term)
FVIII; F8
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Antigen Preparation
"A synthetic peptide corresponding to C-term of the cleavage form around sequence EITRTTLQS of human FVIII protein. It also identical to mouse, chicken origin. "
Background
"The Coagulation Factor VIII (FVIII) is a glycoprotein essential for the intrinsic pathway of blood coagulation. A mature form of FVIII is a single-chain, 2351 amino acid polypeptide with a MW 265kDa. The FVIII can be activated by proteolytic cleavage intracellularly into a two-chain heterodimer, a heavy-chain and light-chain. The development of anti-factor VIII (FVIII) antibodies is currently one of the most serious complications in the treatment of haemophilia A patients due to the nature and properties of anti-FVIII antibodies, their mechanism of action, their neutralization by anti-idiotypic antibodies, and the role of T cells in FVIII inhibitor formation."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
This antibody recognizes ~80 kDa and 265 kDa of human coagulation Factor VIII protein. The other species are not tested
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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