Antigen Preparation
The cell membrane extract from meloma cell lines of SK-MEL.
Background
Anti-HMB45 is a useful melanoma immunohistochemical marker that reacts with antigenspresent on immature melanosomes. Anti-HMB45 is useful for identifying amelanoticmelanoma from other neoplastic lesions with similar morphology. Melanosomes, the pigment granules that provide tissues with colour and photoprotection, are the cellular site of synthesis, storage and transport of melanin pigments. They are synthesised in mammalian skin melanocytes, in choroidal melanocytes and retinal pigment epithelial (RPE) cells in the eye, and in melanophores (a class of pigment-containing cells) in lower vertebrates. Although melanosomes are considered to be lysosome-related organelles, recent data show that their contents derive from early endosomal membranes. Disorders of pigmentation were among the first genetic diseases ever recognized because of their visually striking clinical phenotypes, resulting from defects of pigmentary melanocytes. These diseases may be most usefully considered as abnormalities of melanocyte development, function, or survival.
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
5-10 µg/ml
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