Antigen Preparation
A synthetic peptide containing N-terminus of human histone H3 with citrulline modification at 2/8/17.
Background
"Histones are basic nuclear proteins that together with DNA make up the nucleosome structure in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The Histone 3 citrillination occurs by enzymatic conversion of arginine residue to citrulline. This leads to chromatin relaxation and enables activation of transcription. H3 citrullinations in different arginines plays a central role in release of chromatin from neutrophil extracellular traps (NETs), which have been shown to promote tumor progression and metastasis. It is important in diagnosis, prognosis and therapeutics of cancer and autoimmune disease. This antibody is specific for histone H3 Citrillination at R2, R8 and/or R17. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
5-10 µg/ml
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