Antigen Preparation
"A synthetic peptide corresponding to a segment of the HLA-B. It is identical among human, mouse and rat."
Background
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. MHC class I heavy chains associate with β2-microglobulin and are expressed on nearly all nucleated cells. MHC class I molecules are involved in the presentation of of antigens to CD8+ T cells and thereby play an important role in cell-mediated immune responses and immune surveillance.
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|