Antigen Preparation
A synthetic peptide corresponding to the C-terminus of cytokeratin 17
Background
"Keratins are expressed predominantly in epithelial cells and as members of the intermediate filament family of proteins, they are part of the largest family of cytoskeletal proteins. Cytokeratin 17 is a member of the keratin family, a type I keratin. It is the smallest keratin protein and shows a diverse expression pattern ranging from developing embryo, mature striated muscle, complex and simple epithelia, to epithelial stem cells. It is expressed in mammary gland ductal and secretory cells, gastrointestinal tract, hair follicules, muscle fibers, etc. Keratins are widely used as diagnostic markers to detect tumors in both primary and distal sites and to determine tumors’ tissue of origin in order to aid in treatment strategies. "
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|