Background
"This is monoclonal anti-human IgM HP6083 antibody heavy chain secondary antibody. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. Naive B cells express the transmembrane forms of IgM and IgD on their surface. IgM expression begins as soon as heavy and light chains recombine early in B cell development, and persists until class switch recombination occurs following B cell activation. The heavy and light chains are held together both by disulfide bonds and by non-covalent interactions. The µ heavy chain of IgM is a protein of ~576 amino acids, and includes a variable domain, four distinct constant region domains, and a “tailpieceâ€. The µ heavy chain bears oligosaccharides at five asparagine residues. The spleen is necessary for development and function of IgM memory B cells. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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