Abbomax

Career

CD4

Rat anti CD4 Monoclonal Antibody

Download Product PDF PDF Image

MSDS Download Product PDF

Antigen Preparation

A full length of human CD4 recombinant protein

Background

"CD4 is a glycoprotein consisting of up to five polypeptide chains (gp20, gp26, p16, p20, p28). CD3 is required for proper assembly, trafficking and surface expression of TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. CD3 is involved in T cell activation, proliferation and apoptosis pathway. This protein is present on virtually all peripheral blood T cells and thymocytes. This anti-CD3 antibody is commonly used as a phenotypic marker for human T cells."

PURIFICATION The rat IgG is purified by Protein A-Affinity Chromatography according to Isotyping	SPECIFICITY "This antibody recognizes human, mouse and bacteria CD4 protein. The other species was not tested. "
FORMULATION This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer	STORAGE The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Gene ID

Applications/Suggested Working Dilutions

Western Blot

0.1-1 µg/ml

ELISA

0.01-0.1 µg/ml

Immunoprecipitation

2-5 µg/ml

IHC

2-10 µg/ml

Flow cytometry

0.5-5 µg/106 cells

Order Info

Catalog #:	603-042
Lot #:
Size:	100ug/200ul
Host:	Rat
Clone:	RM4-5
Isotyping:	IgG2a
Applications:	IHC(P), FC
Reactivity:
Price:	$ 245.00

REFERENCES