Antigen Preparation
A synthetic peptide corresponding to the C-terminus of human p16INK4a protein.
Background
"The INK4 (inhibitor of cyclin-dependent kinase 4) family consists of four tumor-suppressor proteins: p15(INK4B), p16(INK4A), p18(INK4C), and p19(INK4D). While their sequences and structures are highly homologous, they show appreciable differences in conformational flexibility, stability, and aggregation tendency. p16 (INK4a, CDKN2A, cyclin dependent kinase inhibitor 2A) is a tumor suppressor gene that inhibits cdk4 and cdk6 which phosphorylate the Rb protein. P16INK4a overexpression has been demonstrated in cervical cancers as a result of functional inactivation of pRb. Thus, this antibody can be an important marker for dysplastic squamous and glandular cells of the cervix."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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