Antigen Preparation
"A synthetic peptide corresponding to c-terminus of GAPDH protein. It is identical among human, mice rat and other species."
Background
"The glyceraldehyde-3-phosphate dehydrogenase protein (GAPDH) is a ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. Aa a “house-keeping†enzyme, it catalyzes the synthesis of 1,3-biphosphogycerate, a “high energy†intermediate used for the synthesis of ATP. It catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). It has been identified to have uracil DNA glycosylase activity in the nucleus. It has a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. It is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. GAPDH has also been found to bind specifically to proteins implicated in pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and huntingtin. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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