MyoD1
myogenic differentiation 1; PUM; MYF3; MYOD; bHLHc1
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Antigen Preparation
"A synthetic peptide EHY SGD SDA SSP RSN corresponding to human MyoD1. It is identical among human, mice and rat."
Background
"Myogenic differentiation 1 (Myo D1), a transcription factor that belongs to the basic helix-loop-helix (HLH)family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody recognizes human MyoD1 protein. It cross reacts to human, mice and rat."
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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