Antigen Preparation
"A synthetic peptide KK ELE DLV RRE HAS IDA QSG corresponding to human SirT-2. It is identical among human, mice and rat."
Background
"SIRTs belongs to sirtuin family proteins. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. Sirt2 is overexpressed during mitosis and is multiply phosphorylated at the G2/M transition of the cell cycle, indicating that it plays a role in the control of mitotic exit in the cell cycle. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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