Antigen Preparation
A mixture of the synthetic peptides of AEAMNYEG-S*(PO3H2)-PIKVTLAT and AEAMNYEGSPIKVTLAT. This sequence is identical to human, mouse, rat.
Background
"Nucleophosmin (NPM) is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. It preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of nucleophosmin is regulated by protein kinase CK2 and promotes the release of denatured substrates from nucleophosmin. The phosphorylation of NPM is associated in its interaction with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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