Nucleophosmin (NPM)Paired S70
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Antigen Preparation
A synthetic peptide corresponding to AEAMNYEGSPIKVTLAT. This sequence is identical to human, mouse, rat.
Background
"Nucleophosmin (NPM) is a member of the nucleophosmin/nucleoplasmin family that shuttles between the nucleus and cytoplasm. This protein regulates the stability and transcriptional activity of p53 and acts as a molecular chaperone. It preferentially binds to denatured proteins and has been shown to stimulate DNA polymerase activity and control the duplication of centrosomes. The chaperone activity of nucleophosmin is regulated by protein kinase CK2 and promotes the release of denatured substrates from nucleophosmin. The phosphorylation of NPM is associated in its interaction with nucleolar proteins including nucleolin, protein P120, HIV-1 Rev protein, and hepatitis delta antigens."
PURIFICATION
The Rabbit IgG is purified by Epitope Affinity Purification
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SPECIFICITY
"This antibody recognizes human Nucleophosmin (NPM)Paired S70 protein without the phosphorylation S70. It cross reacts to human, mice and rat."
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FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
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STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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