Antigen Preparation
A recombinant protein of human NAPRT1 (1-250aa) with FLAG tag.
Background
"NAPRT, nicotinate phosphoribosyltransferase, can convert Nicotinic Acid (NA; niacin) to NA mononucleotide (NaMN), then to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress. This conversion is essential to increase cellular NAD levels, which prevents oxidative stress of the cells. NAPRT is expressed as three isoforms produced by alternative splicing events. The gene that encodes NAPRT maps to human chromosome 8, which makes up nearly 146 million bases and encodes about 800 genes. Translocation of portions of chromosome 8 with amplifications of the c-Myc gene are found in some leukemias and lymphomas, and are typically associated with a poor prognosis. Portions of chromosome 8 have been linked to schizophrenia and bipolar disorder."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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