Antigen Preparation
A synthetic peptide of Epha 3
Background
"This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EphA3 expression is associated with tumor promotion in certain types of cancer; however, it acts as a tumor suppressor in others. EphA3 is up-regulated by epidermal growth factor and promotes formation of glioblastoma cell aggregates. EphA3 inhibits migration and invasion of esophageal cancer cells by activating the mesenchymal epithelial transition process."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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