Antigen Preparation
A recombinant protein of human Cytochrome C
Background
"Cytochrome C is a 15 kDa small heme protein that functions as a central component of the electron transport chain in mitochondria. The protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis . Cytochrome C release upon Fas receptor activation depends on translocation of full-length Bid and the induction of the mitochondrial permeability transition. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Cytochrome c is a highly conserved protein across the spectrum of species, found in plants, animals, and many unicellular organisms."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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