Antigen Preparation
A recombinant protein of human CD75
Background
"This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Aberrant sialylation of glycoproteins has been detected in many tumors, and upregulation of the ST6GAL1 has been implicated with tumor aggressiveness and chemoresistance in experimental models. Studies show the impact of sialyltransferase ST6GAL1 overexpression on different colon cancer cell types.
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Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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