Antigen Preparation
A recombinant protein of human CD49
Background
"CD49e is a 135 kD protein that belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha 5 subunit. This subunit associates with the beta 1 subunit to form a fibronectin receptor (VLA-5). This integrin may promote tumor invasion, and higher expression of this gene may be correlated with shorter survival time in lung cancer patients. It was shown that human CD49e-negative NK cells are tissue-resident in the liver. Integrin α5 is upregulated during chemical-induced osteogenic differentiation of human mesenchymal stem cells and plays a critical role in this process by regulating focal adhesion kinase (FAK)/extracellular-related kinase (ERK) and mitogen-associated protein kinase (MAPK) signaling."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
0.5-5 µg/106 cells
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