Digoxigenin

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Antigen Preparation
A chmically conjugated Digoxigenin
Background
"Digoxigenin is derived from a plant steroid hormone digoxin found in the plants Digitalis sp. Digoxigenin is a hapten, a small molecule with high antigenicity. Anti-digoxigenin antibodies are used in a variety of biological immuno-assays. The antibodies may be labeled with dyes, enzymes or fluorescence, Digoxigenin has been also used successfully in labeling nucleic acids."
PURIFICATION
The mouse IgG is purified by Protein A-Affinity Chromatography according to Isotyping
SPECIFICITY
This antibody recognizes Digoxigenin compond. It is used for testing Digoxigenin conjugation.
FORMULATION
This affinity purified antibody is supplied in sterile Phosphatebuffered saline (pH7.2) containing antibody stabilizer
STORAGE
The antibodies are stable for 24 months from date of receipt when stored at –20oC to –70oC. The antibodies can be stored at 2oC-8oC for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Gene ID
Applications/Suggested Working Dilutions
Western Blot
0.1-1 µg/ml
ELISA
0.01-0.1 µg/ml
Immunoprecipitation
2-5 µg/ml
IHC
2-10 µg/ml
Flow cytometry
Not tested
Order Info
Catalog #: 605-680
Lot #:
Size: 100ug/200ul
Host: Mouse
Clone: Dig
Isotyping: IgG1
Applications: IHC(P), ELISA
Reactivity: Hu
Price: $ 245.00
REFERENCES
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