Antigen Preparation
A synthetic peptide of human Connexin 32
Background
"Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 32, also known as gap junction beta-1 protein (GJB1) is a transmembrane protein. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Disorders are typically characterized by peripheral motor and sensory neuropathy with or without fixed CNS abnormalities and/or acute, self-limited episodes of transient neurologic dysfunction (especially weakness and dysarthria). Defects in assembly of gap junction-forming proteins, are observed in a variety of cancers. The carboxyl tail of connexin32 regulates gap junction assembly in human prostate and pancreatic cancer cells."
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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