Background
Keratins are the intermediate filament proteins that form a dense meshwork of filaments throughout the cytoplasm of epithelial cells. The keratin proteins of epithelial tissues are commonly known as "keratins" or are sometimes referred to as "cytokeratins".The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. As many as six of this type II cytokeratin (KRT6) have been identified; the multiplicity of the genes is attributed to successive gene duplication events. Keratin 6 is found mostly in squamous epithelium. Keratin 6A was the first type II keratin sequence determined. It is found with keratin 16 and/or keratin17 in the palm and sole epidermis, the epithelial cells of the nail bed, the tong, the epithelial lining of oral mucosa and esophagus, and hair follicles. This keratin 6 isoform is thought to be the most abundant of the K6 isoforms. Keratin 6B isoform is thought be less abundant than the closely related to keratin 6A protein. Keratin 6A gene silencing suppresses cell invasion and metastasis of nasopharyngeal carcinoma via the β catenin cascade
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
5-10 µg/ml
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