Antigen Preparation
A recombinant protein of human Nix
Background
"This protein belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The NIX protein possesses the BH3 domain. NIX localizes to mitochondria, interacts with BCL2 and BCL-XL. C-terminal transmembrane domain of NIX, but not its BH3 domain, is essential for its proapoptotic activity. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. NIX is implicated in hypoxia-induced tumor cell death. Stable NIX expression retards the growth of cancer cell lines, suggesting that NIX may be a tumor suppressor. "
Applications/Suggested Working Dilutions
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Immunoprecipitation
2-5 µg/ml
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Flow cytometry
Not tested
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