Antigen Preparation
A synthetic peptide of human beta-catenin
Background
"Beta-catenin is a cytosolic, 88 kDa, 781 amino acid protein belongs to the β-catenin family. βâ€catenin has been shown to perform two apparently unrelated functions: it has a crucial role in cell–cell adhesion in addition to a signaling role as a component of the Wnt/wg pathway. The N-terminus domain, containing the binding site and the phosphorylation sites. Beta-Catenin serves as a link between cytoskeleton actin and transmembrane cadherin(s). It can enter the nucleus and interact with the TCF/LEF family of transcription factors, initiating gene expression. Normally, β-catenin transcriptional activity is suppressed by a Ser/Thr kinase termed GSK3β and/or Casein Kinase I (CK1). Kinases are constitutively active and phosphorylates β-catenin at multiple sites, including S33 and S37, Y96, Y228, Y280 etc. Phosphorylation of β-catenin targets the molecule for degradation via a ubiquination-mediated pathway. GSK3β activity can be blocked by upstream signaling events such as Wnt-Frizzled interaction. This inhibits GSK3β, allowing unphosphorylated β-catenin to enter the nucleus and initiate gene activation. The phosphorylation of beta-catenin might contribute to tumorigenesis. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. "
Applications/Suggested Working Dilutions
|
|
|
Immunoprecipitation
2-5 µg/ml
|
|
Flow cytometry
Not tested
|